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Caspase-9 Antibody (Rat Specific) #9506

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Inquiry Info. # 9506

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    Product Specifications

    REACTIVITY R
    SENSITIVITY Endogenous
    MW (kDa) 17, 38, 40, 51
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Caspase-9 Antibody (Rat Specific) detects endogenous levels of full length (51 kDa) and large fragments of caspase-9. This antibody does not recognize any other cleaved caspases.

    Species Reactivity:

    Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 353 of rat caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

    Alternate Names

    25 kDa caspase-9 dominant negative protein; Apaf3; apoptotic protease activating factor 3; apoptotic protease Mch-6; Casp-9-CTD; Casp9; Casp9_v1; caspase 9, apoptosis-related cysteine peptidase; Caspase 9, isoform CRA_a; Caspase-9; caspase-9 dominant negative form; Caspase-9 long isoform; caspase-9-carboxyl-terminal divergent; Ice-Lap6; Ice-like apoptotic protease 6; Mch6; RNCASP9

    For Research Use Only. Not for Use in Diagnostic Procedures.
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