Render Target: STATIC
Render Timestamp: 2024-10-04T09:48:59.096Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Cathepsin S Antibody #25084

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 25, 37
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cathepsin S Antibody recognizes endogenous levels of total cathepsin S protein. The antibody detects the full-length pro-protein (37 kDa), and proteolytically activated cathepsin S (25 kDa). In some cell extracts, the antibody weakly detects a 70 kDa protein of unknown identity.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly233 of human cathepsin S protein. Antibodies are purified by peptide affinity chromatography.

    Background

    Cathepsin S (CTSS) is a cysteine protease belonging to the cathepsin family (1). It is synthesized as a 37 kDa pro-protein, which is proteolytically processed to remove the amino terminal activation peptide, yielding the mature (active) cathepsin S protein (2). The protein is localized to the lysosomal or endosomal compartments of antigen-presenting cells such as B cells, macrophages, and dendritic cells, where it plays an important role in immune response activation (3,4). Specifically, cathepsin S proteolytically removes the self-associated invariant chain (CD74) from MHC class II, and promotes MHC-exogenous antigenic peptide loading (5). Cathepsin S may also be secreted by cells to the extracellular matrix, where its proteolytic degradation of matrix proteins and cytokines (e.g., collagens, elastin, PAR2) has been shown to influence cell signaling (1). Aberrant expression and activity of cathepsin S have been associated with various disease states, including atherosclerosis, fibrosis and inflammation, autoimmune disease, and cancer (6-10), making it a potential biomarker and a promising therapeutic target.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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