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57219
CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free) #57219

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  1. WB
  2. IHC
Western Blotting Image 1: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CCL5/RANTES (E9S2K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human CCL5/RANTES protein (hCCL5/RANTES; +), using CCL5/RANTES (E9S2K) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 3: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from mouse bone marrow-derived macrophage cells, untreated (-) or treated (+) with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (1 μg/mL, 24 hr), using CCL5/RANTES (E9S2K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 2: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the tonsil using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemistry Image 6: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human small cell carcinoma of the salivary gland using CCL5/RANTES (E9S2K) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or CCL5/RANTES transfected (right), using CCL5/RANTES (E9S2K) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 57219
Cat. # Size Qty. Price
57219SF
100 µg
$ 746

Supporting Data

REACTIVITY H M
SENSITIVITY Endogenous
MW (kDa) 10
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #36467. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #36467.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CCL5/RANTES (E9S2K) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CCL5/RANTES protein. This antibody recognizes human CCL5/RANTES protein and is also reactive with mouse CCL5/RANTES; however, this antibody is not suggested for immunohistochemical analysis of mouse tissues. Non-specific staining was observed in human pancreatic islets by immunohistochemistry.

Species Reactivity:

Human, Mouse

Source / Purification

Monoclonal antibody is produced by immunizing animals with human CCL5/RANTES recombinant protein.

Background

RANTES/CCL5 (regulated upon activation, T cell expressed and secreted) is a member of the "C-C" or β family of chemokines that induce inflammation and are associated with a number of inflammatory disorders (1,2). RANTES is produced and secreted mainly by CD8+ T cells, macrophages, and platelets, as well as epithelial cells, fibroblasts, and some solid tumors (2-7). RANTES acts as a chemoattractant and has other regulatory functions on a number of cell types, including monocytes, memory T cells, NK cells, eosinophils, basophils, dendritic cells, and mast cells (3, 7-9). Signaling by RANTES is mediated by several G protein-coupled receptors (GPCRs), including CCR1, CCR3, CCR4, and CCR5.
  1. Schall, T.J. (1991) Cytokine 3, 165-83.
  2. Schall, T.J. et al. (1988) J Immunol 141, 1018-25.
  3. Kameyoshi, Y. et al. (1992) J Exp Med 176, 587-92.
  4. Rathanaswami, P. et al. (1993) J Biol Chem 268, 5834-9.
  5. Mattei, S. et al. (1994) Int J Cancer 56, 853-7.
  6. Stellato, C. et al. (1995) J Immunol 155, 410-8.
  7. Schall, T.J. et al. (1990) Nature 347, 669-71.
  8. Kuna, P. et al. (1992) J Immunol 149, 636-42.
  9. Mattoli, S. et al. (1995) Biochem Biophys Res Commun 209, 316-21.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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