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CD11c (D1V9Y) Horse Chimeric Monoclonal Antibody #52131

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  • IF

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Horse chimeric IgG4
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CD11c (D1V9Y) Rabbit Monoclonal Antibody #97585.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD11c (D1V9Y) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total CD11c protein. Nonspecific staining of the epithelium in fixed frozen mouse intestine was observed by immunofluorescence. Nonspecific staining in fixed frozen mouse brain was observed by immunofluorescence.

    Species Reactivity:

    Mouse

    Source / Purification

    This recombinant chimeric antibody is engineered from CD11c (D1V9Y) Rabbit Monoclonal Antibody #97585 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, CD11c (D1V9Y) Rabbit Monoclonal Antibody #97585, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1153 of mouse CD11c protein.

    Background

    CD11c (integrin αX, ITGAX) is a transmembrane glycoprotein that forms an α/β heterodimer with CD18 (integrin β2), which interacts with a variety of extracellular matrix molecules and cell surface proteins (1). CD11c is primarily used as a dendritic cell marker. Dendritic cells can be classified into two major types: CD11c+ conventional dendritic cells that specialize in antigen presentation, and CD11c- plasmacytoid dendritic cells that specialize in type I interferon production (2, 3). CD11c expression has also been observed on activated NK cells, subsets of B cells, monocytes, granulocytes, and some B cell malignancies including hairy cell leukemia (4-7).

    Alternate Names

    AI449405; CD11 antigen-like family member C; Cd11c; CD11C (p150) alpha polypeptide; complement receptor 4; Cr4; integrin alpha X; Integrin alpha-X; integrin aX; ITAX; Itgax; leukocyte adhesion glycoprotein p150,95 alpha chain; leukocyte adhesion receptor p150,95; mFLJ00114; MFLJ00114 protein; N418

    For Research Use Only. Not for Use in Diagnostic Procedures.
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