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CD16 (2H7) Mouse Monoclonal Antibody #88251

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  • IHC

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgG2a
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:200 - 1:800
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied as liquid tissue culture supernatant containing sodium azide as a preservative. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

    Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.

    Protocol

    Specificity / Sensitivity

    CD16 (2H7) Mouse Monoclonal Antibody recognizes endogenous levels of CD16 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to the external domain of CD16 protein, common to both the transmembrane form and the GPI-linked form.

    Background

    CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).

    Alternate Names

    CD16; CD16A; CD16a antigen; Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc gamma receptor III-A; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIII-alpha; Fc-gamma RIIIa; FCG3; FCG3A; FcgammaRIIIA; FCGR3; FCGR3A; FCGRIII; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; low affinity immunoglobulin gamma receptor III-a Fc fragment; neutrophil-specific antigen NA

    For Research Use Only. Not for Use in Diagnostic Procedures.
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