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CD162/PSGL-1 (F6N5G) Rabbit Monoclonal Antibody #78211

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  • WB
  • IP
  • IHC
  • F

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 280, 140
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized) 1:800 - 1:3200
    Flow Cytometry (Live) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD162/PSGL-1 (F6N5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD162/PSGL-1 protein. This antibody reacts with monomeric and dimeric forms of CD162/PSGL-1 protein.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of mouse CD162/PSGL-1 protein. This antibody recognizes residues surrounding Leu63 of mouse CD162/PSGL-1 protein.

    Background

    P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is a disulfide-linked homodimer expressed on the cell surface of lymphoid and myeloid cells as a type I transmembrane sialoglycoprotein (1,2). The mature protein backbone of the extracellular domain of PSGL-1 is extensively modified with N-linked glycans, O-linked glycans, sulfated tyrosine residues, and sialyl-Lewis X epitopes (3). Collectively, these modifications modulate high-affinity interactions between PSGL-1 and multiple selectins, thereby facilitating immune cell trafficking to primary and secondary lymphoid organs (4-8).

    Alternate Names

    CD162; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand-1; Psg; PSGL-1; Psgl1; Selectin P ligand; selectin, platelet (p-selectin) ligand; Selp1; Selpl; Selplg

    For Research Use Only. Not for Use in Diagnostic Procedures.
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