Western blot analysis of extracts from various cell lines using CD180/RP105 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CD180/RP105 protein is not detected in either HeLa or MOLT-4 cells.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD180 protein (hCD180-Myc/DDK; +), using CD180/RP105 Antibody (upper), DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
CD180/RP105 Antibody recognizes endogenous levels of total CD180/RP105 protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp483 of human CD180/RP105 protein. Antibodies are purified by peptide affinity chromatography.
CD180/RP105 is a type-I transmembrane glycoprotein that is restricted to expression in mature B-cells. Research studies have shown that CD180 engages signaling cascades in mature B-cells that drive cell proliferation (1-3). Indeed, CD180 functions with TLR4 as an LPS receptor on B-cells and, upon binding LPS, transmits proliferation and survival signals that require the Vav family of guanine nucleotide exchange factors (4-6).
Explore pathways + proteins related to this product.
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Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
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