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20030
CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

CD2 (D6V9F) Rabbit mAb (BSA and Azide Free) #20030

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  1. WB
  2. IHC
Western Blotting Image 1: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using CD2 (D6V9F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using CD2 (D6V9F) Rabbit mAb performed on the Leica® BOND Rx. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human tonsil using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using CD2 (D6V9F) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using CD2 (D6V9F) Rabbit mAb (left) or CD2 mouse mAb (right). These two antibodies detect unique, non-overlapping epitopes on CD2. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded HDLM-2 cell pellet (left, positive) or Raji cell pellet (right, negative) using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human T-cell lymphoma using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: CD2 (D6V9F) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal rhesus monkey spleen using CD2 (D6V9F) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 20030
Cat. # Size Qty. Price
20030SF
100 µg
$ 746

Supporting Data

REACTIVITY H Mk
SENSITIVITY Endogenous
MW (kDa) 48-65
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier-free version of product #9987. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #9987.

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

CD2 (D6V9F) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total CD2 protein. Non-specific staining was observed in skeletal muscle by immunohistochemistry.

Species Reactivity:

Human, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala270 of human CD2 protein.

Background

CD2 is a transmembrane glycoprotein expressed early in thymocyte development and present on most circulating T cells (1,2). CD2 plays a role in T cell adhesion through binding to its ligand CD58 (LFA-3) (3). Stimulation of CD2 also leads to T cell activation and proliferation (2). T cells from mice deficient in both CD2 and CD28 have severe defects in T cell activation and function, while T cells deficient in either CD2 or CD28 are still capable of mounting a response, suggesting that CD2 and CD28 may have overlapping functions and may be able to compensate for each other (4). In addition, engagement of CD2 and CD58 was recently demonstrated to be the primary costimulatory signal in T cells that lack CD28 (5). CD2 expression also distinguishes a subset of plasmacytoid dendritic cells found in tumors and tonsils that express lysozyme, higher levels of IL-12 p40, and higher levels of CD80 (6).
  1. Kamoun, M. et al. (1981) J Exp Med 153, 207-12.
  2. Meuer, S.C. et al. (1984) Cell 36, 897-906.
  3. Selvaraj, P. et al. Nature 326, 400-3.
  4. Green, J.M. et al. (2000) J Immunol 164, 3591-5.
  5. Leitner, J. et al. (2015) J Immunol 195, 477-87.
  6. Matsui, T. et al. (2009) J Immunol 182, 6815-23.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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