CD2AP (A599) Antibody #5478
Inquiry Info. # 5478
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Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CD2AP (A599) Antibody recognizes endogenous levels of total CD2AP protein.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala599 of human CD2AP protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton (1-3). It plays a role in formation of tight junctions in specialized cell types such as the slit diaphragm in the kidney glomerulus (4). CD2AP is also involved in the immunological synapse between CD2 expressing T cells and antigen presenting cells (5). It has been shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR (3).
- Kirsch, K.H. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6211-6216.
- Kirsch, K.H. et al. (2001) J. Biol. Chem. 276, 4957-4963.
- Lynch, D.K. et al. (2003) J. Biol. Chem. 278, 21805-21813.
- Kawachi, H. et al. (2006) Nephrology (Carlton) 11, 274-281.
- Hutchings, N.J. et al. (2003) J. Biol. Chem. 278, 22396-22403.
Limited Uses
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