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CD300a (E6B9T) Rabbit Monoclonal Antibody #87572

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  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 40-70
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD300a (E6B9T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD300a protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala210 of human CD300a protein.

    Background

    CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail (1,2,5). Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granulocytes, mast cells, and subsets of B cells (1-5). Human, but not mouse, CD300a is also expressed by unstimulated NK cells and subsets of T cells (1,5). Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation (1,5).

    Alternate Names

    CD300 antigen-like family member A; CD300A; CD300a antigen; CD300a molecule; CLM-8; CLM8; CMRF-35-H9; CMRF-35H; CMRF35-H; CMRF35-H9; CMRF35-like molecule 8; CMRF35H; CMRF35H leukocyte immunoglobulin-like receptor; CMRF35H9; IGSF12; Immunoglobulin superfamily member 12; Inhibitory receptor protein 60; IRC1; IRC1/IRC2; IRC2; IRp60; leukocyte membrane antigen; MAIR-I; NK inhibitory receptor

    For Research Use Only. Not for Use in Diagnostic Procedures.
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