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CD57 (HNK-1) Mouse mAb #72031

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  • IHC
  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgM
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:400 - 1:1600
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.

    Protocol

    Specificity / Sensitivity

    CD57 (HNK-1) Mouse mAb recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells.

    Background

    CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1).

    Alternate Names

    autostainer; B3GAT1; BOND; Leica; Rx

    For Research Use Only. Not for Use in Diagnostic Procedures.
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