Render Target: STATIC
Render Timestamp: 2024-12-12T10:53:00.905Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-05-10 22:35:42.613
Product last modified at: 2024-09-05T13:30:08.598Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

CD57 (HNK-1) Mouse mAb #72031

Filter:
  • IHC
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Mouse IgM
    Application Key:
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    IHC Leica Bond 1:400 - 1:1600
    Immunohistochemistry (Paraffin) 1:100 - 1:400
    Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200

    Storage

    Supplied in 10 mM PBS containing 0.05% BSA and 0.05% sodium azide. Stable for 36 months when stored at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present, but will not interfere with antibody performance.

    Protocol

    Specificity / Sensitivity

    CD57 (HNK-1) Mouse mAb recognizes the CD57 carbohydrate modification produced by the B3GAT1 protein enzyme.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a membrane antigen from HSB-2 cells.

    Background

    CD57 antigen, also known as HNK-1 and Leu7, is a terminally sulfated glycan carbohydrate epitope (glyco-epitope) expressed on a variety of proteins, lipids, and chondroitin sulfate proteoglycans at the cell surface (1,2). CD57 is synthesized by the enzyme B3GAT1 and is present on a subset of peripheral blood lymphocytes, including NK cells and CD8+ T cells, as well as neural cells and striated muscle (3-5). Studies have shown that CD57 is not expressed on platelets, monocytes, granulocytes, or red blood cells (6). The CD57 epitope may play a role in neural cell adhesion (7), and characterizes unique maturation states in T and NK cells (8,9). CD57 is an important marker when studying functional immune deficiency in patients with autoimmune diseases, infectious diseases, and cancers (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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