CD59 Antibody #65055
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CD59 Antibody recognizes endogenous levels of total CD59 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln99 of human CD59 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
CD59 is a GPI-anchored membrane protein that functions as an inhibitor of the complement membrane attack complex (MAC). CD59 binds to complement components C8 and C9, preventing C9 polymerization and insertion into membranes, therefore inhibiting the complement-dependent cytolysis (CDC) (1). CD59 is a ubiquitously expressed cell membrane protein that protects cells from CDC. Rare cases of CD59 deficiency have been reported to cause paroxysmal nocturnal hemoglobinuria in human patients (2,3). Expression of CD59 on tumor cells and viral infected cells makes them resist antibody-dependent complement-mediated lysis. Potent inhibitors for CD59 have been actively pursued for therapeutic applications (4,5). In addition, CD59 may regulate insulin secretion by modulating exocytosis (6).
- Sugita, Y. and Masuho, Y. (1995) Immunotechnology 1, 157-68.
- Mevorach, D. (2015) Mol Immunol 67, 51-5.
- Höchsmann, B. and Schrezenmeier, H. (2015) Hematol Oncol Clin North Am 29, 495-507.
- Cai, B. et al. (2014) J Biol Chem 289, 12109-25.
- Lan, J. et al. (2014) J Immunol 193, 3577-89.
- Krus, U. et al. (2014) Cell Metab 19, 883-90.
Limited Uses
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