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  3. CD8 alpha (D8A8Y) Feline Chimeric Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD8 alpha (D8A8Y) Feline Chimeric Monoclonal Antibody #61120

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  • IHC

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Feline chimeric IgG1
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as CD8 alpha (D8A8Y) Rabbit Monoclonal Antibody #85336.

    Product Usage Information

    Application Dilution
    Immunohistochemistry (Paraffin) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD8 alpha (D8A8Y) Feline Chimeric Monoclonal Antibody recognizes endogenous levels of total CD8α protein. Monkey species reactivity is immunohistochemistry (Paraffin) only.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    This recombinant chimeric antibody is engineered from CD8 alpha (D8A8Y) Rabbit Monoclonal Antibody #85336 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit antibody, but contains a feline-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, CD8 alpha (D8A8Y) Rabbit Monoclonal Antibody #85336, is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD8α protein.

    Background

    Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

    Alternate Names

    CD8; CD8 antigen alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8A; CD8a molecule; Leu2; Leu2 T-lymphocyte antigen; MAL; OKT8 T-cell antigen; p32; T cell co-receptor; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

    Database Links

    UniProt ID: P01732


    Entrez-Gene Id: 925


    Limited Uses

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    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
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