Render Target: SSR
Render Timestamp:
6/3/2026, 6:54:37 AM EDT
6/3/2026, 10:54:37 AM UTC
Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2026-06-03 05:09:43.058
Product last modified at: 2026-06-03T08:00:24.399Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CD8 alpha (F6F3L) Rabbit Monoclonal Antibody #40345

Filter:
  • WB
  • IHC
  • IF
  • F

    Product Specifications

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 35-42
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:200 - 1:800
    Immunofluorescence (Frozen) 1:200 - 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400
    Flow Cytometry (Live) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CD8 alpha (F6F3L) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD8 alpha protein. This antibody is suitable for use in paraffin-embedded patient-derived xenograft (PDx) models.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to N-terminal mouse CD8 alpha protein. This antibody recognizes residues surrounding Asn150 of mouse CD8 alpha protein.

    Background

    Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

    Alternate Names

    BB154331; CD8 antigen, alpha chain; Cd8a; Ly-; Ly-2; Ly-3; Ly-35; Ly-B; Lyt-; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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