Immunohistochemical analysis of paraffin-embedded human appendix using CD8α (C8/144B) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lymph node using CD8α (C8/144B) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using CD8α (C8/144B) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human spleen using CD8α (C8/144B) Mouse mAb.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For EDTA: Heat slides in a microwave submersed in 1X EDTA unmasking solution until boiling is initiated; follow with 15 min at a sub-boiling temperature (95°-98°C). No cooling is necessary.
posted February 2015
revised March 2016
Protocol Id: 664
CD8α (C8/144B) Mouse mAb (IHC Specific) recognizes endogenous levels of total CD8 protein.Species Reactivity:
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD8 protein.
Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
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