CDK7 (MO1) Mouse mAb #2916
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | H R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Mouse IgG2b |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:2000 |
| Immunoprecipitation | 1:200 |
| Immunohistochemistry (Paraffin) | 1:500 |
Storage
Protocol
Specificity / Sensitivity
CDK7 (MO1) Mouse mAb detects endogenous levels of total CDK7 protein. The antibody does not cross-react with other CDKs.
Species Reactivity:
Human, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CDK7. The epitope corresponds to a region surrounding Gln320 of human CDK7.
Background
CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5).
Limited Uses
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