CENP-F (D6X4L) Rabbit mAb #58982
- WB
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 450 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CENP-F (D6X4L) Rabbit mAb recognizes endogenous levels of total CENP-F protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1794 of human CENP-F protein.
Background
CENP-F (mitosin), is a kinetochore-associated protein whose expression and localization to chromatin is regulated in a cell cycle-dependent manner, with its highest expression in G2/M phases (1, 2). CENP-F is required for appropriate localization of other kinetocore-associated proteins, including CENP-E. CENP-F regulates kinetocore function and maintenance of the mitotic spindle checkpoint. Farnesylation of CENP-F is required for its localization and function (3). CENP-F also interacts with the mitochondrial protein, miro, to direct the distribution of mitochondria to daughter cells as they exit mitosis (4). Researchers have shown that CENP-F drives prostate tumor growth synergistically with FOXM1 in human and mouse (5), and that the gene for CENP-F is among those frequently amplified in hepatocellular, head and neck, and esophageal carcinomas (6-8). CENP-F expression has also been shown in research studies to be associated with poor prognosis in breast cancer (9).
Limited Uses
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