CHMP2A Antibody #84706
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28, 31 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CHMP2A Antibody recognizes endogenous levels of total CHMP2A protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CHMP2A protein. Antibodies are purified by peptide affinity chromatography.
Background
CHMP2A is a component of ESCRT-III (endosomal sorting complex required for transport-III) (1,2). The ESCRT system is comprised of multiple protein complexes denoted as ESCRT-0, -I, -II, and -III, and VPS4 ATPase that are recruited to various cellular membranes and function fission reactions (3,4). Yeast contains four core ESCRT-III subunits: Vps20, Snf7, Vps24, and Vps2. Humans share these subunits and additionally have four Snf7 paralogs (CHMP4A-D) and two Vps2 paralogs (CHMP2A and B). The ESCRT-III complex comprising CHMP2A is involved in various membrane events, including multivesicular bodies, viral budding, cytokinesis, nuclear membrane reformation, and phagophore closure during autophagy and mitophagy (5-9).
- Shim, S. et al. (2007) Traffic 8, 1068-79.
- Lata, S. et al. (2009) Biochem Soc Trans 37, 156-60.
- McCullough, J. et al. (2013) Annu Rev Biochem 82, 663-92.
- Henne, W.M. et al. (2013) Cold Spring Harb Perspect Biol 5, a016766.
- Takahashi, Y. et al. (2018) Nat Commun 9, 2855.
- Zhen, Y. et al. (2020) Autophagy 16, 826-841.
- Lafaurie-Janvore, J. et al. (2013) Science 339, 1625-9.
- Agromayor, M. and Martin-Serrano, J. (2006) J Biol Chem 281, 23083-91.
- Azmi, I.F. et al. (2008) Dev Cell 14, 50-61.
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