Product Information

Kit Usage Information

Protocols

4170: Western Blotting
4241: Western Blotting, Immunoprecipitation (Magnetic)
4351: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic
6505: Western Blotting, Immunoprecipitation (Magnetic)
7074: Western Blotting
11891: Western Blotting, Immunoprecipitation (Magnetic)
11912: Western Blotting, Immunoprecipitation (Magnetic)

Product Description

The Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit provides an economical means to investigate the CHD family members associated with the histone deacetylase NuRD complex. This kit contains enough primary antibody to perform four western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit recognizes total endogenous levels of the specific target protein. CHD8 (D3C1) Rabbit mAb also cross-reacts with a protein of unknown origin at 140 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, and CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.

Database Links

UniProt ID: Q12873-3 , Q14839 , Q9HCK8 , O14647 , O14646 , Q9P2D1

Entrez-Gene Id: 1107 , 1108 , 57680 , 1106 , 1105 , 55636

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.

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Chromodomain Helicase DNA-Binding (CHD) Family Antibody Sampler Kit #12775