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  3. CK1 alpha (F1T9E) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

CK1 alpha (F1T9E) Rabbit Monoclonal Antibody #95920

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 34
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CK1 alpha (F1T9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total CK1 alpha protein. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with other CK1 proteins.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys329 of human CK1 alpha protein.

    Background

    Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (alpha, alpha prime, beta, gamma 1-3, delta, and epsilon) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1 epsilon (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes, including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of beta-Catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).

    Alternate Names

    casein kinase 1 alpha 1; casein kinase 1, alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI-alpha; CKIa; clock regulator kinase; CSNK1A1; down-regulated in lung cancer; epididymis secretory sperm binding protein Li 77p; HEL-S-77p; HLCDGP1; KC1A; PRO2975

    Database Links

    UniProt ID: P48729


    Entrez-Gene Id: 1452


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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