CK1δ Antibody #12417
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 44 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CK1δ protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes, including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).
CK1δ is involved in many cellular processes such as differentiation (5), cell growth and apoptosis (6-8), and control of the circadian rhythm (9).
- Gross, S.D. and Anderson, R.A. (1998) Cell Signal 10, 699-711.
- Vancura, A. et al. (1994) J Biol Chem 269, 19271-8.
- Gietzen, K.F. and Virshup, D.M. (1999) J Biol Chem 274, 32063-70.
- Polakis, P. (2002) Curr Biol 12, R499-R501.
- Bryja, V. et al. (2007) J Cell Sci 120, 586-95.
- Knippschild, U. et al. (2005) Onkologie 28, 508-14.
- Knippschild, U. et al. (1997) Oncogene 15, 1727-36.
- Brockschmidt, C. et al. (2008) Gut 57, 799-806.
- Vanselow, K. and Kramer, A. (2007) Cold Spring Harb Symp Quant Biol 72, 167-76.
Limited Uses
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