CLASP2 (D5K3E) Rabbit mAb #14629
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 165 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Human cytoplasmic linker-associate proteins 1 and 2 (CLASP1 and CLASP2) are evolutionarily conserved proteins that localize to the plus ends of interphase microtubules. During mitosis, CLASP 1 and CLASP2 localize to the centrosomes and kinetochores (KT) where they regulate mitotic spindle positioning to ensure proper chromosome alignment (2,3). Research studies indicate that phosphorylation of the carboxy terminus of CLASP2 during mitosis by CDK1 and PLK1 is required for efficient mitotic MT-KT attachment (4). Phosphorylation of CLASP2 at Ser1013 is a critical step that primes CLASP2 for further phosphorylation by PLK1 (4). The additional phosphorylation of CLASP2 at Ser533 and Ser537 by GSK3-3β controls the distribution of CLASP2 on MTs by inhibiting CLASP2 interaction with the Rac1/cdc42 effector protein IQGAP1 (5).
Limited Uses
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