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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HDAC1 (D5C6U) XP® Rabbit mAb 34589 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Chromatin Immunoprecipitation
H M R Mk 62 Rabbit IgG
HDAC2 (D6S5P) Rabbit mAb 57156 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Chromatin Immunoprecipitation
H M R Mk 60 Rabbit IgG
HDAC3 (D2O1K) Rabbit mAb 85057 20 µl
Western Blotting Immunoprecipitation Chromatin Immunoprecipitation
H M R Mk 49 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Class I HDAC Antibody Sampler Kit provides an economical means of detecting Class I HDAC proteins using control antibodies against HDAC1, HDAC2 and HDAC3. The kit contains enough primary antibodies to perform at least two western blot experiments.

Specificity / Sensitivity

Each antibody in the Class I HDAC Antibody Sampler Kit detects endogenous levels of its target protein. Cross-reactivity was not observed with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to Ala440 of human HDAC1, His438 of human HDAC3, and Gly416 of human HDAC3 proteins.

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

1.  Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.

2.  Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.

3.  Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.

4.  Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.

5.  Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.

6.  Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

7.  Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

Entrez-Gene Id 3065 , 3066 , 8841
Swiss-Prot Acc. Q13547 , Q92769 , O15379

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

Class I HDAC Antibody Sampler Kit