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79891
Class II HDAC Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Class II HDAC Antibody Sampler Kit #79891

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Class II HDAC Antibody Sampler Kit: Image 1

Western blot analysis of extracts from various cell lines using HDAC4 (D8T3Q) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 2

Immunoprecipitation of HDAC5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HDAC5 (D1J7V) Rabbit mAb. Western blot analysis was performed using HDAC5 (D1J7V) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 3

Western blot analysis of extracts from HeLa and Jurkat cells using HDAC6 (D21B10) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 4

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC7 (D4E1L) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 5

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Class II HDAC Antibody Sampler Kit: Image 6

Western blot analysis of extracts from various cell lines using HDAC5 (D1J7V) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 7

Western blot analysis of extracts from C2C12 cells, wild-type mouse embryonic fibroblasts (MEFs), and HDAC6 (-/-) MEFs using HDAC6 (D21B10) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).

Class II HDAC Antibody Sampler Kit: Image 8

Immunohistochemical analysis of paraffin-embedded K-562 (left) and LNCaP (right) cell pellets using HDAC7 (D4E1L) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HDAC7 (D4E1L) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Class II HDAC Antibody Sampler Kit: Image 10

Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 (D4E1L) Rabbit mAb.

Class II HDAC Antibody Sampler Kit: Image 11

Western blot analysis of extracts from various cell lines using HDAC7 (D4E1L) Rabbit mAb.

To Purchase # 79891T
Product # Size Price
79891T
1 Kit  (4 x 20 µl) $ 327

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HDAC4 (D8T3Q) Rabbit mAb 15164 20 µl
  • WB
  • IP
H M R Mk 140 Rabbit IgG
HDAC5 (D1J7V) Rabbit mAb 20458 20 µl
  • WB
  • IP
H M R Mk 140 Rabbit IgG
HDAC6 (D21B10) Rabbit mAb 7612 20 µl
  • WB
  • IP
H M R 160 Rabbit IgG
HDAC7 (D4E1L) Rabbit mAb 33418 20 µl
  • WB
  • IHC
H M Mk 124 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Class II HDAC Antibody Sampler Kit provides an economical means of detecting Class II HDAC proteins using control antibodies against HDAC4, HDAC5, HDAC6, and HDAC7. The kit contains enough primary antibodies to perform at least two western blot experiments.

Specificity / Sensitivity

Each antibody in the Class II HDAC Antibody Sampler Kit detects endogenous levels of its target protein. Cross-reactivity was not observed with other family members.

Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of human HDAC4, Leu445 of human HDAC5, the carboxy terminus of HDAC6, and Leu66 of HDAC7 proteins.

Background

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

  1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.
  2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.
  3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.
  4. Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.
  5. Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.
  6. Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.
  7. Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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