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PhosphoSitePlus® Resource

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
HDAC4 (D8T3Q) Rabbit mAb 15164 20 µl
Western Blotting Immunoprecipitation
H M R Mk 140 Rabbit IgG
HDAC5 (D1J7V) Rabbit mAb 20458 20 µl
Western Blotting Immunoprecipitation
H M R Mk 140 Rabbit IgG
HDAC6 (D21B10) Rabbit mAb 7612 20 µl
Western Blotting Immunoprecipitation
H M R 160 Rabbit IgG
HDAC7 (D4E1L) Rabbit mAb 33418 20 µl
Western Blotting Immunohistochemistry
H M Mk 124 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Class II HDAC Antibody Sampler Kit provides an economical means of detecting Class II HDAC proteins using control antibodies against HDAC4, HDAC5, HDAC6, and HDAC7. The kit contains enough primary antibodies to perform at least two western blot experiments.


Specificity / Sensitivity

Each antibody in the Class II HDAC Antibody Sampler Kit detects endogenous levels of its target protein. Cross-reactivity was not observed with other family members.


Source / Purification

Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of human HDAC4, Leu445 of human HDAC5, the carboxy terminus of HDAC6, and Leu66 of HDAC7 proteins.

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).


1.  Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.

2.  Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.

3.  Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.

4.  Cress, W.D. and Seto, E. (2000) J Cell Physiol 184, 1-16.

5.  Gray, S.G. and Ekström, T.J. (2001) Exp Cell Res 262, 75-83.

6.  Thiagalingam, S. et al. (2003) Ann. N.Y. Acad. Sci. 983, 84-100.

7.  Vigushin, D.M. and Coombes, R.C. (2004) Curr Cancer Drug Targets 4, 205-18.


Entrez-Gene Id 9759 , 10014 , 10013 , 51564
Swiss-Prot Acc. P56524 , Q9UQL6 , Q9UBN7 , Q8WUI4


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

79891
Class II HDAC Antibody Sampler Kit