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  • Additional protein information
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 9664 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17, 19 Rabbit IgG
Cleaved Caspase-6 (Asp162) Antibody 9761 20 µl
Western Blotting
H M R 18 Rabbit 
Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 8438 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R 18 Rabbit IgG
Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 7237 20 µl
Western Blotting Immunoprecipitation
H 37 Rabbit IgG
Cleaved Caspase-9 (Asp315) Antibody (Human Specific) 9505 20 µl
Western Blotting Immunoprecipitation
H 35 Rabbit 
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb 5625 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 89 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Cleaved Caspase Antibody Sampler Kit provides an economical means to evaluate the activation status of caspases by detecting their cleaved forms. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each primary antibody.

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175), Cleaved Caspase-6 (Asp162), Cleaved Caspase-7 (Asp198), Cleaved Caspase-9 (Asp315), Cleaved Caspase-9 (Asp330), and Cleaved PARP (Asp214) Antibodies detect endogenous levels of the large cleavage fragments of their respective targets. These antibodies will not cross-react with their respective full-length proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3 or to residues surrounding Asp214 in human PARP, Asp330 of human caspase-9, or Asp198 of human caspase-7. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Asp315 of human caspase-9 or to the carboxy-terminal sequence of the large subunit (p18) of rat caspase-6. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).

1.  Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.

2.  Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.

3.  Nakagawa, T. et al. (2000) Nature 403, 98-103.

4.  Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.

5.  Li, F. et al. (1998) Nature 396, 580-584.

6.  Du, C. et al. (2000) Cell 102, 33-42.

Entrez-Gene Id 836 , 839 , 840 , 842 , 142
Swiss-Prot Acc. P42574 , P55212 , P55210 , P55211 , P09874

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

Cleaved Caspase Antibody Sampler Kit