Render Target: STATIC
Render Timestamp: 2024-12-09T11:19:26.286Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-04-05 20:42:59.154
Product last modified at: 2024-12-04T18:15:09.737Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Cleaved PARP (Asp214) (7C9) Mouse mAb #9548

Filter:
  • WB

    Supporting Data

    REACTIVITY M
    SENSITIVITY Endogenous
    MW (kDa) 89
    Source/Isotype Mouse IgG2b
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cleaved PARP (Asp214) (7C9) Mouse mAb detects endogenous levels of the large fragment of PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

    Species Reactivity:

    Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP.

    Background

    PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA-binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

    (This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)
    For Research Use Only. Not For Use In Diagnostic Procedures.
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