CLIC4 (D2A7D) Rabbit mAb #12644
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 28 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Chloride intracellular channel 4 (CLIC4) is a well-studied member of the chloride intracellular channel family. Expression of CLIC4 is regulated by p53 and c-Myc, and CLIC4 is required for p53 and c-Myc-mediated apoptosis in some cell types (6,7). CLIC4 contributes to TNF-α mediated apoptosis independent of NF-κB (8). Moreover, CLIC4 regulates the maturation of keratinocytes, differentiation of adipocytes and blood vessel lumen formation (9-12). Research studies show that CLIC4 translocates from the cytoplasm to the nucleus in response to cellular stress (13). In addition, nuclear CLIC4 enhances TGF-β signaling (14). In human cancer tissues, CLIC4 is excluded from the nucleus of tumor cells and its expression is reduced in tumor epithelial tissues (15); conversely, CLIC4 expression is markedly elevated in the adjacent stroma of multiple human cancers. Researchers have shown that high stromal CLIC4 protein levels enhance tumor invasiveness and progression (16).
- Littler, D.R. et al. (2010) FEBS Lett 584, 2093-101.
- Oakley, A.J. (2005) Curr Opin Struct Biol 15, 716-23.
- Littler, D.R. et al. (2005) FEBS J 272, 4996-5007.
- Singh, H. and Ashley, R.H. (2006) Biophys J 90, 1628-38.
- Suh, K.S. et al. (2004) J Biol Chem 279, 4632-41.
- Fernández-Salas, E. et al. (2002) Mol Cell Biol 22, 3610-20.
- Shiio, Y. et al. (2006) J Biol Chem 281, 2750-6.
- Suh, K.S. et al. (2005) Cancer Res 65, 562-71.
- Kitamura, A. et al. (2001) Biochem Biophys Res Commun 287, 435-9.
- Bohman, S. et al. (2005) J Biol Chem 280, 42397-404.
- Suh, K.S. et al. (2007) J Cell Sci 120, 2631-40.
- Ulmasov, B. et al. (2009) Am J Pathol 174, 1084-96.
- Malik, M. et al. (2010) J Biol Chem 285, 23818-28.
- Shukla, A. et al. (2009) Nat Cell Biol 11, 777-84.
- Suh, K.S. et al. (2007) Clin Cancer Res 13, 121-31.
- Shukla, A. et al. (2013) Oncogene , [ePub ahead of print].
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