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84336
COL1A1 Antibody
Primary Antibodies
Polyclonal Antibody

COL1A1 Antibody #84336

Citations (16)

We recommend the following alternatives

# Product Name Application Reactivity
  • WB
  • IP
  • IF
H
  • WB
  • IP
H M R
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Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 220
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

COL1A1 Antibody recognizes endogenous levels of total COL1A1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1197 of human COL1A1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and posttranslational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11).

  1. Prockop, D.J. and Kivirikko, K.I. (1995) Annu Rev Biochem 64, 403-34.
  2. Chang, S.W. et al. (2012) Biophys J 102, 640-8.
  3. Zhang, Y. and Stefanovic, B. (2016) Int J Mol Sci 17, 419.
  4. Parsons, C.J. et al. (2011) J Biol Chem 286, 8609-19.
  5. Cai, L. et al. (2010) J Mol Biol 395, 309-26.
  6. Jimenez, S.A. and Saitta, B. (1999) Springer Semin Immunopathol 21, 397-414.
  7. Forlino, A. et al. (2011) Nat Rev Endocrinol 7, 540-57.
  8. Li, J. et al. (2016) World J Surg Oncol 14, 297.
  9. Oleksiewicz, U. et al. (2017) J Cancer Res Clin Oncol 143, 1133-41.
  10. Barcus, C.E. et al. (2017) Breast Cancer Res 19, 9.
  11. Jolly, L.A. et al. (2016) Cancer Res 76, 1804-13.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
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