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COL1A1 (E8F4L) Horse Chimeric Monoclonal Antibody #94924

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  • IF

    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Horse chimeric IgG4
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as COL1A1 (E8F4L) Rabbit Monoclonal Antibody #72026.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    COL1A1 (E8F4L) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total COL1A1 protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    This recombinant chimeric antibody is engineered from COL1A1 (E8F4L) Rabbit Monoclonal Antibody #72026 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody, but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, COL1A1 (E8F4L) Rabbit Monoclonal Antibody #72026, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1196 of human COL1A1 protein.

    Background

    Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex composed of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and post-translational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11).

    Alternate Names

    Alpha-1 type I collagen; alpha1(I) procollagen; CAFYD; CO1A1; COL1A1; collagen 1; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; collagen I; collagen of skin, tendon and bone, alpha-1 chain; collagen type I; collagen type I alpha 1 chain; collagen, type I, alpha 1; EDSARTH1; EDSC; OI1; OI2; OI3; OI4; pro-alpha-1 collagen type 1; type I collagen; type I proalpha 1; type I procollagen alpha 1 chain

    For Research Use Only. Not for Use in Diagnostic Procedures.
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