PhosphoPlus® CREB (Ser133) Antibody Kit #9190

Product Description

The PhosphoPlus CREB (Ser133) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of CREB at Ser133.

Specificity / Sensitivity

Phospho-CREB (Ser133) (87G3) Rabbit mAb detects endogenous levels of CREB only when phosphorylated at Ser133. This antibody also detects the phosphorylated form of CREB-related protein ATF-1. CREB (48H2) Rabbit mAb detects endogenous levels of total CREB protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB (Phospho-CREB (Ser133) (87G3) Rabbit mAb), or with a synthetic peptide corresponding to residues near the carboxy-terminus of human CREB (CREB (48H2) Rabbit mAb).

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca²⁺, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

Pathways & Proteins

Explore pathways + proteins related to this product.

Select Your Pathway/Protein B Cell Receptor Signaling ErbB/HER Signaling Growth And Differentiation Control by MAPKs Inhibition of Apoptosis Parkinson's Disease Regulation of P38 MAPKs T Cell Receptor Signaling Protein: P16220

Database Links

Swiss-Prot Acc.: P16220

Entrez-Gene Id: 1385

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