CTCF Antibody #2899
- WB
- IP
- IF
- ChIP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
- ChIP-Chromatin Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
For optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Chromatin IP | 1:25 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
- Klenova, E.M. et al. (2002) Semin Cancer Biol 12, 399-414.
- Klenova, E.M. et al. (1993) Mol Cell Biol 13, 7612-24.
- Loukinov, D.I. et al. (2002) Proc Natl Acad Sci USA 99, 6806-11.
- Mukhopadhyay, R. et al. (2004) Genome Res 14, 1594-602.
- Hark, A.T. et al. (2000) Nature 405, 486-9.
- Ohta, T. et al. (1999) Am J Hum Genet 64, 397-413.
- Chao, W. et al. (2002) Science 295, 345-7.
- Yu, W. et al. (2004) Nat Genet 36, 1105-10.
- El-Kady, A. and Klenova, E. (2005) FEBS Lett 579, 1424-34.
- Filippova, G.N. et al. (1998) Genes Chromosomes Cancer 22, 26-36.
- Filippova, G.N. et al. (2002) Cancer Res 62, 48-52.
- Vatolin, S. et al. (2005) Cancer Res 65, 7751-62.
- Hong, J.A. et al. (2005) Cancer Res 65, 7763-74.
- D'Arcy, V. et al. (2008) Br J Cancer 98, 571-9.
Limited Uses
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