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CUL4A (F3F6U) Rabbit Monoclonal Antibody #65732

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  • WB
  • IP

    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 82, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CUL4A (F3F6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total CUL4A protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse CUL4A protein.

    Background

    Cullin-4 (CUL4) is a member of the cullin family of related ubiquitin ligases (1). A pair of mammalian CUL4 proteins have been identified: CUL4A and CUL4B. The carboxy-terminal CUL4 domain interacts with Rbx1 and E2 enzymes, while the amino-terminal CUL4 domain interacts with the BPB domain of UV-damaged DNA-binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex (2). This CUL4-DDB1 complex binds a variety of WD40-containing proteins that help to enhance additional substrate recruitment (2,3). The CUL4 complex has been shown to target substrates involved in DNA repair, cell cycle progression (2), transcription (3), and development (2-6).

    Alternate Names

    2810470J21Rik; AW495282; CUL-4A; Cul4a; cullin 4A; Cullin-4A; MGC36573

    For Research Use Only. Not for Use in Diagnostic Procedures.
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