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CXCL10 (F9N8I) Rabbit Monoclonal Antibody (BSA and Azide Free) #33892

Filter:
  • IHC

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa)
    Source/Isotype Rabbit IgG
    Application Key:
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    This product is the carrier free version of product #45008. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

    This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

    BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

    Formulation

    Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

    For standard formulation of this product see product #45008

    Storage

    Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

    Specificity / Sensitivity

    CXCL10 (F9N8I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CXCL10 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CXCL10 protein.

    Background

    C-X-C motif chemokine ligand 10 (CXCL10, IP-10) is a chemokine produced by several cell types and induced in response to pro-inflammatory cytokines, such as IFN-gamma and TNF-alpha (1,2). CXCL10 binds to the chemokine receptor CXCR3 and induces calcium flux and cell migration (3). The CXCR3 protein is normally expressed on activated CD4+ T cells and NK cells, which can produce pro-inflammatory cytokines, including IFN-gamma and TNF-alpha (3,4). As a result, these molecules act in an amplification loop to promote immune responses in the contexts of both autoimmunity and a tumor immune response (5,6).

    Alternate Names

    10 kDa interferon gamma-induced protein; C-X-C motif chemokine 10; C-X-C motif chemokine ligand 10; C7; chemokine (C-X-C motif) ligand 10; crg-2; CXCL10; CXCL10(1-73); CXL10; gamma IP10; Gamma-IP10; gIP-10; IFI10; INP10; interferon-inducible cytokine IP-10; IP-10; IP10; mob-1; protein 10 from interferon (gamma)-induced cell line; SCYB10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10

    For Research Use Only. Not for Use in Diagnostic Procedures.
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