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CXXC1 Antibody #12585

Filter:
  • WB

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 82
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    CXXC1 Antibody recognizes endogenous levels of total CXXC1 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of human CXXC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine Cxxc1 gene results in early embryonic lethality while Cxxc1-null embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation in vitro following withdrawal of leukemia inhibitory factor LIF (6).

    Alternate Names

    2410002I16Rik; 5830420C16Rik; CFP1; CGBP; CpG binding protein; CpG-binding protein; CXXC finger 1 (PHD domain); CXXC finger protein 1; CXXC-type zinc finger protein 1; CXXC1; DNA-binding protein with PHD finger and CXXC domain; hCGBP; HsT2645; PCCX1; PHD finger and CXXC domain-containing protein 1; PHF18; SPP1; ZCGPC1; zinc finger, CpG binding-type containing 1

    For Research Use Only. Not for Use in Diagnostic Procedures.
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