Render Target: STATIC
Render Timestamp: 2025-02-12T12:09:26.441Z
Commit: 7500bcdc731e9059bbdfbdbe9e72caa896e426e8
XML generation date: 2024-04-05 20:32:49.771
Product last modified at: 2025-02-10T20:15:09.695Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

Cyclin A2 (BF683) Mouse mAb #4656

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 55
    Source/Isotype Mouse IgE
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cyclin A2 (BF683) Mouse mAb detects endogenous levels of cyclin A2 and does not cross-react with other cyclins or Cyclin A1.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin A2 protein.

    Background

    While overcoming the G1/S checkpoint to commence DNA replication requires cyclin E, and traversing the G2/M checkpoint to initiate mitosis requires cyclin B to be present, cyclin A seems to be required for both S-phase and M-phase (1). A number of studies have described the ability of overexpressed cyclin A to accelerate the G1 to S transition, causing DNA replication, and cyclin A antisense DNA can prevent DNA replication (2-4). Cyclin A availability is apparently the rate-limiting step for entry into mitosis, and cyclin A is required for the completion of prophase (5). At late prophase, cyclin A may no longer be necessary as cdc2/cyclinB1 becomes active (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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