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Cyclin D3 (DCS22) Mouse Monoclonal Antibody

Cyclin D3 (DCS22) Mouse Monoclonal Antibody #2936

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Product Specifications

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	31
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IHC-Immunohistochemistry

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:2000
Immunohistochemistry (Paraffin)	1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #99580.

Protocol

Specificity / Sensitivity

Cyclin D3 (DCS22) Mouse Monoclonal Antibody detects endogenous levels of total cyclin D3 protein. This antibody does not cross-react with cyclin D1 or cyclin D2.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human cyclin D3 corresponding to residues 241-260.

Background

Activity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4).
Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers (5).

Alternate Names

CCND3; cyclin D3; D3-type cyclin; G1/S-specific cyclin D3; G1/S-specific cyclin-D3

Database Links

UniProt ID: P30281

Entrez-Gene Id: 896

Limited Uses

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