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9384
Cytokeratin Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cytokeratin Antibody Sampler Kit #9384

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Cytokeratin Antibody Sampler Kit: Image 1

Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.

Cytokeratin Antibody Sampler Kit: Image 2

Flow cytometric analysis of MCF-7 cells, using Keratin 8/18 (C51) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

Cytokeratin Antibody Sampler Kit: Image 3

Flow cytometric analysis of Jurkat cells (blue) and HT-29 cells (green) using Keratin 18 (DC10) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Cytokeratin Antibody Sampler Kit: Image 4

Immunhistochemical analysis of paraffin-embedded human breast carcinoma using Keratin 19 (BA17) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 5

Flow cytometric analysis of A431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Cytokeratin Antibody Sampler Kit: Image 6

Flow cytometric analysis of Jurkat cells (blue) and HeLa cells (green) using Keratin 7 (D1E4) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Cytokeratin Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Cytokeratin Antibody Sampler Kit: Image 8

Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytokeratin Antibody Sampler Kit: Image 9

Confocal immunofluorescent analysis of HeLa cells using Keratin 8/18 (C51) Mouse mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

Cytokeratin Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytokeratin Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded MCF-7 cells (positive, left) and A-431 cells (negative, right), using Keratin 19 (BA17) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 12

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Cytokeratin Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Keratin 7 (D1E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytokeratin Antibody Sampler Kit: Image 14

Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.

Cytokeratin Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 17

Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb. As expected, the protein is absent in A-431 and HeLa cells.

Cytokeratin Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 19

Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected.

Cytokeratin Antibody Sampler Kit: Image 20

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 27

Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 31

Western blot analysis of extracts from various cell lines, using Keratin 8/18 (C51) Mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Cytokeratin Antibody Sampler Kit: Image 35

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

To Purchase # 9384T
Product # Size Price
9384T
1 Kit  (6 x 20 µl) $ 458

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Keratin 8/18 (C51) Mouse mAb 4546 20 µl
  • WB
  • IHC
  • IF
  • F
H Mk 46 Keratin 18. 55 Keratin 8. Mouse IgG1
Keratin 18 (DC10) Mouse mAb 4548 20 µl
  • WB
  • IHC
  • IF
  • F
H 46 Mouse IgG1
Keratin 19 (BA17) Mouse mAb 4558 20 µl
  • WB
  • IHC
H 40 Mouse IgG1
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
Keratin 7 (D1E4) XP® Rabbit mAb 4465 20 µl
  • WB
  • IF
  • F
H 52 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

MW (kDa) N/A

Specificity / Sensitivity

The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.

Source / Purification

Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 (D1E4) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 7 protein and Keratin 17 (D73C7) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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