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9384
Cytokeratin Antibody Sampler Kit

Cytokeratin Antibody Sampler Kit #9384

Western Blotting Image 1

Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.

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Western Blotting Image 2

Western blot analysis of extracts from various cell lines, using Keratin 8/18 (C51) Mouse mAb.

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Western Blotting Image 3

Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.

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Western Blotting Image 4

Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb. As expected, the protein is absent in A-431 and HeLa cells.

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Western Blotting Image 5

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

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Western Blotting Image 6

Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected.

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

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Flow Cytometry Image 8

Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.

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IHC-P (paraffin) Image 9

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Keratin 8/18 (C51) Mouse mAb.

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IHC-P (paraffin) Image 10

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.

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IHC-P (paraffin) Image 11

Immunhistochemical analysis of paraffin-embedded human breast carcinoma using Keratin 19 (BA17) Mouse mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

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Flow Cytometry Image 13

Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Keratin 7 (D1E4) XP® Rabbit mAb.

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IF-IC Image 14

Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 15

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Keratin 8/18 (C51) Mouse mAb.

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IHC-P (paraffin) Image 16

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded MCF-7 cells (positive, left) and A-431 cells (negative, right), using Keratin 19 (BA17) Mouse mAb.

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IF-IC Image 19

Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Keratin 7 (D1E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 20

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung, using Keratin 8/18 (C51) Mouse mAb.

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IHC-P (paraffin) Image 21

Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Keratin 8/18 (C51) Mouse mAb.

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Flow Cytometry Image 24

Flow cytometric analysis of HeLa cells, using Keratin 18 (DC10) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

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IHC-P (paraffin) Image 25

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 26

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Keratin 8/18 (C51) Mouse mAb.

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IF-IC Image 27

Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 28

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

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Flow Cytometry Image 29

Flow cytometric analysis of MCF-7 cells, using Keratin 8/18 (C51) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

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Flow Cytometry Image 30

Flow cytometric analysis of MCF7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 31

Confocal immunofluorescent analysis of HeLa cells using Keratin 8/18 (C51) Mouse mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

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IF-IC Image 32

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

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IF-P Image 33

Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Keratin 8/18 (C51) Mouse mAb 4546 20 µl
  • WB
  • IHC
  • IF
  • F
H Mk 46 Keratin 18. 55 Keratin 8. Mouse IgG1
Keratin 18 (DC10) Mouse mAb 4548 20 µl
  • WB
  • IHC
  • IF
  • F
H 46 Mouse IgG1
Keratin 19 (BA17) Mouse mAb 4558 20 µl
  • WB
  • IHC
H 40 Mouse IgG1
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
Keratin 7 (D1E4) XP® Rabbit mAb 4465 20 µl
  • WB
  • IF
  • F
H 52 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.

Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 (D1E4) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 7 protein and Keratin 17 (D73C7) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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