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8614
Cytoskeletal Marker Antibody Sampler Kit

Cytoskeletal Marker Antibody Sampler Kit #8614

 Image 1

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb #8457.

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Western Blotting Image 2

Western blot analysis of extracts from C2C12 cells, rat heart and human heart using Desmin (D93F5) XP® Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.

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Western Blotting Image 4

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

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Western Blotting Image 5

Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.

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IF-IC Image 6

Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Western Blotting Image 7

Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb.

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Western Blotting Image 8

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb.

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Western Blotting Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IF-IC Image 10

Confocal immunofluorescent analysis of C2C12 cells (left) and mouse heart tissue (right) using Desmin (D93F5) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ 5® #4084 (fluorescent DNA dye).

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Flow Cytometry Image 11

Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.

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IHC-P (paraffin) Image 12

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 13

Immunohistochemical analysis of paraffin-embedded human glioblastoma using β-Tubulin (9F3) Rabbit mAb.

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IHC-P (paraffin) Image 14

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb.

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Flow Cytometry Image 15

Flow cytometric analysis of NIH/3T3 cells using β-Actin (D6A8) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

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IF-IC Image 16

Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 17

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 18

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (9F3) Rabbit mAb.

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IHC-P (paraffin) Image 19

Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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IF-IC Image 20

Confocal immunofluorescent analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IHC-P (paraffin) Image 21

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 22

Immunohistochemical analysis of paraffin-embedded human melanoma using β-Tubulin (9F3) Rabbit mAb.

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IHC-P (paraffin) Image 23

Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb.

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IHC-P (paraffin) Image 24

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

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IHC-P (paraffin) Image 25

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).

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Flow Cytometry Image 26

Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

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IHC-P (paraffin) Image 27

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

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Flow Cytometry Image 28

Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

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Flow Cytometry Image 29

Flow cytometric analysis of MCF7 cells, using Pan-Keratin (C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).

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IF-IC Image 30

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-IC Image 31

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

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IF-P Image 32

Confocal immunofluorescent image of paraffin-embedded human colon carcinoma labeled with Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Desmin (D93F5) XP® Rabbit mAb 5332 20 µl
  • WB
  • IF
H M R 53 Rabbit IgG
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
β-Tubulin (9F3) Rabbit mAb 2128 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk Z B 55 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb 5741 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 57 Rabbit IgG
β-Actin (D6A8) Rabbit mAb 8457 20 µl
  • WB
  • IF
  • F
H M R Mk Dm Z 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform two western blots per primary antibody.

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).

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  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  3. Moll, R. et al. (1982) Cell 31, 11-24.
  4. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.
  5. Leader, M. et al. (1987) Histopathology 11, 63-72.
  6. Li, Z. et al. (1996) Dev Biol 175, 362-6.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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