Western blot analysis of extracts from MKN-45 cells, untreated (-) or treated with Forskolin #3828 (30 μM, 20 min; +), or nonphospho-DARPP-32 and phospho-DARPP-32 (Thr34) recombinant proteins using Phospho-DARPP-32 (Thr34) (D27A4) Rabbit mAb (upper) or DARPP-32 (19A3) Rabbit mAb #2306 (lower).
Confocal immunofluorescent analysis of normal rat cerebellum, retina, and ciliary epithelium using DARPP-32 (19A3) Rabbit mAb (green) and Neurofilament-H (RMdO 20) Mouse mAb #2836 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded mouse brain using DARPP-32 (19A3) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded rat retina using DARPP-32 (19A3) Rabbit mAb.
Western blot analysis of extracts from rat and mouse brain using DARPP-32 (19A3) Rabbit mAb.
Western blot analysis of extracts from rat, mouse brain, wild-type mouse striatum and DARPP-32 knock-out mouse striatum, using DARPP-32 (19A3) Rabbit mAb (wild-type mouse striatum and DARPP-32 knock-out mouse striatum was kindly provided by Dr. Paul Greengard and Helen Bateup, Rockefeller University, New York, NY).
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.