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DFF45/DFF35 Antibody #9732

Filter:
  • WB
  • IP

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 35, 45
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DFF45/DFF35 Antibody detects endogenous levels of total full length DFF45 and/or full length DFF35. This antibody does not cross-react with related proteins.

    Species Reactivity:

    Human

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp224 of human DFF45. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases, including caspase-3, -6, -7, -8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and release of its carboxy-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).

    Alternate Names

    DFF-45; DFF1; DFF45; DFFA; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD

    For Research Use Only. Not for Use in Diagnostic Procedures.
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