DNA Methylation Antibody Sampler Kit #88548
Product Information
Kit Usage Information
Protocols
- 5032: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence, Flow Triton Permeabilization (Rabbit), ChIP Magnetic
- 7074: Western Blotting
- 49768: Western Blotting, Immunoprecipitation (Agarose), ChIP Magnetic, Chromatin IP-seq
- 57868: Western Blotting, Immunoprecipitation (Magnetic), Immunofluorescence, Flow, ChIP Magnetic, CUT&RUN Assay, CUT&Tag
Product Description
Specificity / Sensitivity
Each antibody in the DNA Methylation Antibody Sampler Kit detects endogenous levels of its target protein. DNMT3A (E9P2F) Rabbit mAb detects multiple isoforms of DNMT3A, including isoforms 1 and 2, and does not cross-react with DNMT3B or other DNMT proteins. DNMT3B (E8A8A) XP® Rabbit mAb does not cross-react with DNMT3A or DNMT1 protein.
Source / Purification
Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu985 of human DNMT1, the amino terminus of human DNMT3B, and recombinant protein specific to the carboxy terminus of human DNMT3A.
Background
Methylation of DNA at cytosine residues in mammalian cells is a heritable, epigenetic modification that is critical for proper regulation of gene expression, genomic imprinting and development (1,2). Three families of mammalian DNA methyltransferases have been identified: DNMT1, DNMT2, and DNMT3 (1,2). DNMT1 is constitutively expressed in proliferating cells and functions as a maintenance methyltransferase, transferring proper methylation patterns to newly synthesized DNA during replication. DNMT3A and DNMT3B are strongly expressed in embryonic stem cells with reduced expression in adult somatic tissues. DNMT3A and DNMT3B function as de novo methyltransferases that methylate previously unmethylated regions of DNA. DNMT2 is expressed at low levels in adult somatic tissues and its inactivation affects neither de novo nor maintenance DNA methylation. DNMT1, DNMT3A, and DNMT3B together form a protein complex that interacts with histone deacetylases (HDAC1, HDAC2, Sin3A), transcriptional repressor proteins (RB, TAZ-1), and heterochromatin proteins (HP1, SUV39H1) to maintain proper levels of DNA methylation and facilitate gene silencing (3-8). Improper DNA methylation contributes to diseased states such as cancer (1,2). Hypermethylation of promoter CpG islands within tumor suppressor genes correlates with gene silencing and the development of cancer. In addition, hypomethylation of bulk genomic DNA correlates with and may contribute to the onset of cancer. DNMT1, DNMT3A, and DNMT3B are overexpressed in many cancers, including acute and chronic myelogenous leukemias, in addition to colon, breast, and stomach carcinomas (9-12).
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- Kanai, Y. et al. (2001) Int. J. Cancer 91, 205-12.
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