Western blot analysis of extracts from various cell lines and mouse and rat brain using Drebrin (pan) Antibody (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Absence of detectable Drebrin expression in A-431 cells is expected.
|REACTIVITY||H M R Mk|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
Drebrin (pan) Antibody recognizes endogenous levels of total DrebrinE and DrebrinA proteins.Species Reactivity:
Human, Mouse, Rat, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu580 of human Drebrin protein. Antibodies are purified by protein A and peptide affinity chromatography.
Developmentally-regulated brain proteins (Drebrins) are cytoplasmic proteins that were originally identified in the brain as F-actin-binding proteins. There are two mammalian isoforms: adult type (A) and embryonic type (E). These isoforms are derived from a single gene through alternative RNA splicing mechanisms (1). Drebrin E has been observed to accumulate in the developmental stage of migrating neurons and in the growing cell processes of neurons. Drebrin A is found at the dendritic spines of mature cortical neurons where it plays a role in synaptic plasticity (2,3). Although drebrins are primarily found in neurons, they have also been found in skeletal muscle, heart, pancreas, and kidney. Research studies have shown that reduced expression of drebrin in the brain could be associated with Alzheimer’s Disease, Down Syndrome (4), and bipolar disorders (5).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.