Cat. # | Size | Qty. | Price |
---|---|---|---|
76651L | 200 µg |
|
REACTIVITY | All |
SENSITIVITY | Endogenous |
MW (kDa) | |
Source/Isotype | Mouse IgG2a kappa |
Product Information
Application | Dilution |
---|---|
Immunofluorescence (Immunocytochemistry) | 1:500 - 1:1000 |
NOTE: Some CST antibodies work optimally using an alternate protocol. Please see the protocol on the product web page for product-specific recommendations.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: All subsequent incubations should be carried out at room temperature (20-25°C) unless noted otherwise.
NOTE: When including fluorescent cellular dyes in your experiment (DNA dyes, etc.), please refer to the dye product page for its recommended protocol. View our listing of cellular dyes validated for use in immunofluorescence.
Protocol Id: 2685
All Species Expected
Monoclonal antibody is produced by immunizing animals with a nucleic acid sequence.
Double-stranded RNA (dsRNA) is produced during the replication cycle of a broad range of viruses and can trigger the innate immune response. dsRNA is present if cells are infected with a dsRNA virus or it can be generated during the course of single-stranded RNA (ssRNA) virus replication. Host cells do not produce dsRNA so the innate immune response can differentiate between host and viral RNAs by the presence of dsRNA (1). The innate immune response is controlled by several classes of germline-encoded pattern-recognition receptors (PRRs), including retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), Toll-like receptors (TLRs), and NOD-like receptors (NLRs) that sense viral components, such as dsRNA, ssRNA, and DNA (2,3). Activation of PRRs by dsRNA leads to production of type I interferons (IFNs) and proinflammatory cytokines, triggering the adaptive immune response (2,3). Understanding how antiviral responses are initiated, the counter strategies that viruses adopt, and exploring the viral life cycle gives researchers a better comprehension of how to treat infections of different viral diseases (4,5).
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