|Source/Isotype||Mouse IgG2a kappa|
|Immunofluorescence (Immunocytochemistry)||1:500 - 1:1000|
Supplied in a PBS solution that does not contain any stabilizers or preservatives and has been lyophilized. The lyophilized antibody is stable for 24 months when stored at -20°C. Reconstitute using 200 μL of sterile distilled water to create a 1 mg/mL solution. A slight precipitate may be present, but will not interfere with antibody performance. Recommended to centrifuge the reconstituted antibody and use the supernatant. Once reconstituted, this product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles.
NOTE: Some CST antibodies work optimally using an alternate protocol. Please see the protocol on the product web page for product-specific recommendations.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: All subsequent incubations should be carried out at room temperature (20-25°C) unless noted otherwise.
NOTE: When including fluorescent cellular dyes in your experiment (DNA dyes, etc.), please refer to the dye product page for its recommended protocol. View our listing of cellular dyes validated for use in immunofluorescence.
Protocol Id: 2685
All Species Expected
Monoclonal antibody is produced by immunizing animals with a nucleic acid sequence.
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