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3058
DUSP6/MKP3 Antibody
Primary Antibodies

DUSP6/MKP3 Antibody #3058

Reviews ()
Citations (8)

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# Product Name Application Reactivity
  • WB
H

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 42
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

DUSP6/MKP3 Antibody detects endogenous levels of total DUSP6 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human DUSP6. Antibodies are purified by protein A and peptide affinity chromatography.

Background

MAP kinases are inactivated by dual-specificity protein phosphatases (DUSPs) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli, and cellular localization. DUSPs, also known as MAPK phosphatases (MKP), specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (DUSP1-10, DUSP14, DUSP16, and DUSP22) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for DUSP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses, and metabolic homeostasis (5). In addition, research studies have implicated DUSPs in the development of cancer and the response of cancer cells to chemotherapy (6).

DUSP6 specifically dephosphorylates ERK MAP kinase (7).

  1. Camps, M. et al. (2000) FASEB J 14, 6-16.
  2. Theodosiou, A. and Ashworth, A. (2002) Genome Biol 3, REVIEWS3009.
  3. Salojin, K. and Oravecz, T. (2007) J Leukoc Biol 81, 860-9.
  4. Tanoue, T. et al. (2002) J Biol Chem 277, 22942-9.
  5. Dickinson, R.J. and Keyse, S.M. (2006) J Cell Sci 119, 4607-15.
  6. Wu, G.S. (2007) Cancer Metastasis Rev 26, 579-85.
  7. Kim, Y. et al. (2003) Biochemistry 42, 15197-207.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

To Purchase # 3058