Dvl3 Antibody #3218
- WB
- IP
Supporting Data
| REACTIVITY | H M R Hm Mk Mi B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 88 to 93 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
- Mi-Mink
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
Dvl3 Antibody detects endogenous levels of total Dvl3 protein. This antibody does not cross-react with Dvl2.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Mink, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the carboxy terminus of human Dvl3. The antibodies are purified by protein A and peptide affinity chromatography.
Background
Dishevelled (Dsh) proteins are important intermediates of Wnt signaling pathways. Dsh inhibits glycogen synthase kinase-3β promoting β-catenin stabilization. Dsh proteins also participate in the planar cell polarity pathway by acting through JNK (1,2). There are three Dsh homologs, Dvl1, Dvl2 and Dvl3 in mammals. Upon treatment with Wnt proteins, Dvls become hyperphosphorylated (3) and accumulate in the nucleus (4). Dvl proteins also associate with actin fibers and cytoplasmic vesicular membranes (5) and mediate endocytosis of the Fzd receptor after Wnt protein stimulation (6). Overexpression of Dvl has been reported in certain cancers (7,8).
Limited Uses
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