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eEF1A (D10A5) Rabbit Monoclonal Antibody

eEF1A (D10A5) Rabbit Monoclonal Antibody #3586

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Product Specifications

REACTIVITY	H M R Mk
SENSITIVITY	Endogenous
MW (kDa)	50
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat
Mk-Monkey

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

eEF1A (D10A5) Rabbit Monoclonal Antibody detects endogenous levels of total eEF1A protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu60 of human eEF1A protein.

Background

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

Alternate Names

CCS-3; CCS3; cervical cancer suppressor 3; CTCL tumor antigen; EE1A1; EEF-1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a-like protein; EF1A1; elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; epididymis secretory sperm binding protein; Eukaryotic elongation factor 1 A-1; eukaryotic translation elongation factor 1 alpha 1; eukaryotic translation elongation factor 1 alpha 1-like 14; FLJ25721; glucocorticoid receptor AF-1 specific elongation factor; GRAF-1EF; HNGC:16303; LENG7; leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; MGC102687; MGC131894; MGC16224; prostate tumor-inducing protein 1; PTI1; translation elongation factor 1 alpha 1-like 14

Database Links

UniProt ID: P68104

Entrez-Gene Id: 1915

Limited Uses

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