Interested in promotions? | Click here >>
12690
Effector Caspases and Substrates Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Effector Caspases and Substrates Antibody Sampler Kit #12690

Citations (0)
No image available
To Purchase # 12690

Product Description

The Effector Caspases and Substrates Antibody Sampler Kit provides an economical means to evaluate the activation of effector (executioner) caspases. The kit contains enough primary antibody to perform at least four western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Effector Caspases and Substrates Antibody Sampler Kit recognizes endogenous levels of its respective target. Caspase-3 (8G10) Rabbit mAb recognizes full-length (35 kDa) and the large fragment (17/19 kDa) of caspase-3 resulting from cleavage at Asp175. Caspase-6 Antibody recognizes full length (35 kDa) and the small subunit (15 kDa) of caspase-6 resulting from cleavage at Asp193. Caspase-7 (D2Q3L) Rabbit mAb recognizes full-length (35 kDa) and the large subunit (20 kDa) of caspase-7 resulting from cleavage at Asp198. PARP Antibody recognizes full length (116 kDa) and the large fragment (89 kDa) of PARP1 resulting from caspase cleavage at Asp214. Lamin A/C (4C11) Mouse mAb recognizes full-length lamin A and lamin C proteins, and the larger fragments of lamin A (50 kDa) and lamin C (41 kDa) resulting from caspase cleavage. Lamin B1 (D4Q4Z) Rabbit mAb recognizes endogenous levels of total lamin B1 protein and a 25 kDa fragment resulting from caspase cleavage.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 in human caspase-3 protein, residues surrounding Pro158 of human caspase-7 protein, residues surrounding Leu118 of human lamin B1 protein, or with a recombinant fragment of human lamin A protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of caspase-6 or the caspase cleavage site in PARP. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Background

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1), Caspase-6 (Mch2), and Caspase-7 (CMH-1, Mch3, ICE-LAP3) are effector caspases functioning in cellular apoptotic processes (1-6). Upon apoptotic stimulation, initiator caspases such as caspase-9 (ICE-LAP6, Mch6) are cleaved and activated (7). The activated upstream caspases further process downstream executioner caspases by cleaving them into activated large and small subunits, thereby initiating a caspase cascade leading to apoptosis (4,6,8-10).

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (11). This protein can be cleaved by many ICE-like caspases in vitro (1,12) and is one of the main cleavage targets of caspase-3 in vivo (10,13). In human PARP, cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (10,12). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (14).

Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (15-17). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (18,19). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (20-22). Lamin B1 is also cleaved by caspases during apoptosis (23).

  1. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  2. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  3. Faleiro, L. et al. (1997) EMBO J 16, 2271-81.
  4. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
  5. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
  6. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
  7. Li, P. et al. (1997) Cell 91, 479-489.
  8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
  9. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
  10. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  11. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  12. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  13. Tewari, M. et al. (1995) Cell 81, 801-809.
  14. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.
  15. Dunbar, J.C. and Lu, H. (2000) Brain Res Bull 52, 123-6.
  16. Goldberg, M. et al. (1999) Crit Rev Eukaryot Gene Expr 9, 285-93.
  17. Yabuki, M. et al. (1999) Physiol Chem Phys Med NMR 31, 77-84.
  18. Rao, L. et al. (1996) J Cell Biol 135, 1441-55.
  19. Orth, K. et al. (1996) J Biol Chem 271, 16443-6.
  20. Biamonti, G. et al. (1992) Mol Cell Biol 12, 3499-506.
  21. Lin, F. and Worman, H.J. (1995) Genomics 27, 230-6.
  22. Pollard, K.M. et al. (1990) Mol Cell Biol 10, 2164-75.
  23. Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.