Product Information
MW (kDa) | n/a |
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: Do not allow slides to dry at any time during this procedure.
Deparaffinization/Rehydration:
Antigen Unmasking:
NOTE: Consult protocol on product webpage for specific recommendation for the unmasking solution.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid, light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted July 2010
revised August 2011
Protocol Id: 444
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
NOTE: Cells should be grown, treated, fixed, and stained directly in multi-well plates, chamber slides, or on coverslips.
NOTE: All subsequent incubations should be carried out at room temperature unless otherwise noted in a humid, light-tight box or covered dish/plate to prevent drying and fluorochrome fading.
posted July 2010
revised August 2011
Protocol Id: 424
Human, Monkey
Monoclonal antibodies were produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase and Tyr1068 of human EGF receptor, or with a fusion protein containing the cytoplasmic domain of human EGF receptor.
The epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane receptor tyrosine kinase that belongs to the HER/ErbB protein family that includes HER2/ErbB2/neu, HER3/ErbB3, and HER4/ErbB4 (1,2). Ligand binding results in receptor homo- and heterodimerization which stimulates its intrinsic tyrosine kinase activity. Autophosphorylation at residues Tyr992, Tyr1045, Tyr1068, Tyr1148, and Tyr1173, as well as c-Src mediated phosphorylation at Tyr845 and Tyr1101 promote docking of SH2 domain-bearing signaling proteins resulting in cellular responses to EGFR activation (2-6). For example, phosphorylation of Tyr1068 facilitates recruitment of Grb2 which, via association with Sos, stimulates the GTP binding activity of Ras, leading to the activation of MAP kinase and other signaling cascades (7). Following activation, EGFR is rapidly endocytosed and either recycled back to the plasma membrane or targeted for lysosomal degradation (8). Dysregulation of EGFR signaling through activating mutations or gene amplification has been implicated in the pathogenesis of many human malignancies, leading to intense clinical study of this pathway (9-13).
Explore pathways related to this product.
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